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Cytotherapy. 2014 Jun;16(6):734-49. doi: 10.1016/j.jcyt.2014.01.004

Intra-renal arterial injection of autologous bone marrow mesenchymal stromal cells ameliorates cisplatin-induced acute kidney injury in a rhesus Macaque mulatta monkey model

Moghadasali R1, Azarnia M2, Hajinasrollah M3, Arghani H4, Nassiri SM5, Molazem M6, Vosough A7, Mohitmafi S8, Najarasl M3, Ajdari Z3, Yazdi RS9, Bagheri M9, Ghanaati H10, Rafiei B10, Gheisari Y11, Baharvand H12, Aghdami N13
PMID: 24801377

BACKGROUND:

Clinically, acute kidney injury (AKI) is a potentially devastating condition for which no specific therapy improves efficacy of the repair process. Bone marrow mesenchymal stromal cells (BM-MSCs) are proven to be beneficial for the renal repair process after AKI in different experimental rodent models, but their efficacy in large animals and humans remains unknown. This study aims to assess the effect of autologous rhesus Macaque mulatta monkey BM-MSC transplantation in cisplatin-induced AKI.

METHODS:

We chose a model of AKI induced by intravenous administration of 5 mg/kg cisplatin. BM-MSCs were transplanted through intra-arterial injection. The animals were followed for survival, biochemistry analysis and pathology.

RESULTS:

Transplantation of 5 × 10(6) cells/kg ameliorated renal function during the first week, as shown by significantly lower serum creatinine and urea values and higher urine creatinine and urea clearance without hyponatremia, hyperkalemia, proteinuria and polyuria up to 84 d compared with the vehicle and control groups. The superparamagnetic iron oxide nanoparticle-labeled cells were found in both the glomeruli and tubules. BM-MSCs markedly accelerated Foxp3+ T-regulatory cells in response to cisplatin-induced damage, as revealed by higher numbers of Foxp3+ cells within the tubuli of these monkeys compared with cisplatin-treated monkeys in the control and vehicle groups.

CONCLUSIONS:

These data demonstrate that BM-MSCs in this unique large-animal model of cisplatin-induced AKI exhibited recovery and protective properties.

Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

KEYWORDS:

acute kidney injury; bone marrow mesenchymal stromal cells; cisplatin; intra-renal arterial injection

Publication Date: 5/8/2014